ABSTRACT
<p><b>OBJECTIVE</b>The B7-H1/PD-1 co-signaling pathway has recently been found to play a pivotal role in the immune evasion of tumor cells from host immune system. The aim of this study was to examine the B7-H1 and PD-1 expression and TILs status in gastric cancer and to elucidate the clinical relevance of B7-H1 and PD-1 to the pathogenesis of gastric carcinoma.</p><p><b>METHODS</b>Immunohistochemistry and ANAE histochemical staining were used to investigate the in situ expression of B7-H1 and PD-1 and TILs status in the gastric tissues. RT-PCR was used to explore B7-H1 and PD-1 expression at the transcriptional level. The B7-H1 expression at protein level was detected by Western blot.</p><p><b>RESULTS</b>Expression of B7-H1 and PD-1 was found to be increased in gastric carcinoma, but absent in normal gastric tissue. B7-H1 expression in gastric carcinoma was inversely correlated with TILs infiltration. B7-H1 but not PD-1 expression in tumor tissue was significantly correlated with some clinicopathhological variables including depth of invasion, lymph node metastasis and distant metastasis.</p><p><b>CONCLUSION</b>B7-H1 and PD-1 expressions are increased in gastric carcinoma. This signaling pathway may inhibit antitumor immune responses in gastric carcinoma. B7-H1 expression plays a critical role in the pathogenesis of human gastric carcinoma,and might be a promising prognostic marker and therapeutic target in the treatment of this disease.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Antigens, CD , Genetics , Metabolism , Apoptosis Regulatory Proteins , Genetics , Metabolism , B7-H1 Antigen , CD4-Positive T-Lymphocytes , Allergy and Immunology , Lymphatic Metastasis , Lymphocyte Subsets , Allergy and Immunology , Lymphocytes, Tumor-Infiltrating , Allergy and Immunology , Neoplasm Invasiveness , Neoplasm Metastasis , Neoplasm Staging , Programmed Cell Death 1 Receptor , RNA, Messenger , Metabolism , Stomach Neoplasms , Genetics , Allergy and Immunology , PathologyABSTRACT
Objective To analyze the concentration of epithelial cell derived neutrophil-activating protein 78(CXCL-5) in the sy-(novial) fluid(SF) and peripheral blood in children with rheumatoid arthritis(RA) and evaluate its clinical relevance to the disease development.Methods SF and peripheral sera were taken from 26 children with RA and 16 healthy controls,and CXCL-5 was measured by enzyme linked immunosorbent assay(ELISA).CXCL-5 in SF was also measured in RA children after receiving steroid treatment.Results The levels of CXCL-5 was significantly higher than that in the SF of RA children compared with healthy controls(P0.05).Interestingly,CXCL-5 markedly decreased in RA children after steroid treatment(P